Engage and Scan

Engage

	Select Microscope > Engage or click the Engage icon on the toolbar. A pre-engage check begins, followed by Z-stage motor motion.
	After engaging, it may be necessary to recheck the Cantilever Tune parameters: Select Microscope > Cantilever Tune or click the Tune icon in the Workflow Toolbar. In the Tip offset box enter a value between 50 and 200 nm and click OK. Adjust the Drive frequency if necessary.

Optimize Scan Parameters

Withdraw the Tip

	Select RealTime > Withdraw or click the Withdraw icon in the Workflow Toolbar. The SPM stops scanning, and ascends to the sample clearance height defined in the SPM Parameters menu.
	Replace or move the sample.
	Use the Focus Surface command to move the SPM up if you desire more clearance between the tip and sample.

CAUTION: Never withdraw samples without verifying that the tip has adequate clearance during the entire sample removal sequence.

Previous steps:

1. Set Up the Experiment
2. Scanner, Probe, and Sample Preparation
3. Locate the Probe Tip
4. Tune the Cantilever
5. Focus Surface
6. Check Initial Scan Parameters

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